1C8S
BACTERIORHODOPSIN D96N LATE M STATE INTERMEDIATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.631, 60.631, 108.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| R-factor | 0.172 |
| R-free | 0.21900 |
| Structure solution method | ISOMORPHOUS |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.330 |
| Total number of observations | 110517 * | |
| Number of reflections | 14622 | |
| <I/σ(I)> | 19.2 | 2.1 |
| Completeness [%] | 95.0 | 98.1 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 5.6 | 20 * | Gabriele, R., (1998) J. Struct. Biol., 121, 82. * |
| 1 | other * | 5.6 | 20 * | Gabriele, R., (1998) J. Struct. Biol., 121, 82. * |
| 1 | other * | 5.6 | 20 * | Gabriele, R., (1998) J. Struct. Biol., 121, 82. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3.3 (mg/ml) | |
| 2 | 1 | 1 | sodium potassium Pi | 1 (M) | |
| 3 | 1 | 1 | MPD | 2.5 (%) |
