1C8D
CANINE PANLEUKOPENIA VIRUS EMPTY CAPSID STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-15 |
| Detector | FUJI |
| Spacegroup name | P 1 |
| Unit cell lengths | 267.563, 268.446, 274.328 |
| Unit cell angles | 61.95, 62.62, 60.19 |
Refinement procedure
| Resolution | 9.000 - 3.000 |
| R-factor | 0.214 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.588 |
| Phasing software | GLRF |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.090 |
| Number of reflections | 322866 |
| Completeness [%] | 31.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 19 * | 0.75-1.5% PEG8000, 20 mM TRIS pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 10 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris | 20 (mM) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 0.75-1.50 (%) | |
| 5 | 1 | reservoir | 8 (mM) | or 5mM EDTA |
