1C7O

CRYSTAL STRUCTURE OF CYSTALYSIN FROM TREPONEMA DENTICOLA CONTAINS A PYRIDOXAL 5'-PHOSPHATE-L-AMINOETHOXYVINYLGLYCINE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-09-25
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	89.373, 107.929, 176.156
Unit cell angles	90.00, 90.17, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.201
Rwork	0.193
R-free	0.26000
RMSD bond length	0.007 *
RMSD bond angle	1.400 *
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.046	0.204
Total number of observations	149605 *
Number of reflections	106874
<I/σ(I)>	28.9
Completeness [%]	92.4	87
Redundancy	1.5	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	20 *	PEG 4000, magnesium acetate, MES, pH 6.5, VAPOR DIFFUSION/SITTING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/v))
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	MES-NaOH	100 (mM)