1C4Z

STRUCTURE OF AN E6AP-UBCH7 COMPLEX: INSIGHTS INTO THE UBIQUITINATION PATHWAY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	110
Detector technology	CCD
Collection date	1999-09-03
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	103.400, 112.700, 123.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.234
Rwork	0.237
R-free	0.29000
RMSD bond length	0.011 *
RMSD bond angle	1.707
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.055	0.413
Total number of observations	106238 *
Number of reflections	42425
<I/σ(I)>	17
Completeness [%]	89.8 *	89.5
Redundancy	2.5	2.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG1500, MAGNESIUM CHLORIDE, MES-NA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	32 (mg/ml)
2	1	reservoir	PEG1500	10 (%(w/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	sodium MES	0.1 (M)	pH6.5