1C4X

2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (BPHD) FROM RHODOCOCCUS SP. STRAIN RHA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	I 4 2 2
Unit cell lengths	110.800, 110.800, 136.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.400
R-factor	0.178
Rwork	0.178
R-free	0.22700
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	1.300
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	CCP4
Refinement software	X-PLOR (2.4)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.400
Rmerge	0.063
Number of reflections	15274
Completeness [%]	92.4
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5