1C4F

GREEN FLUORESCENT PROTEIN S65T AT PH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	51.934, 62.898, 69.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 2.250
Rwork	0.193
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.011
RMSD bond angle	20.197 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	TNT (5F)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.300
High resolution limit [Å]	2.250
Rmerge	0.058
Number of reflections	14685
Completeness [%]	96.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6		10-13% PEG 3400, 100MM SODIUM ACETATE AND 100 MM AMMONIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	PEG3400	10-13 (%)
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir	ammonium acetate	100 (mM)