1C3V
DIHYDRODIPICOLINATE REDUCTASE FROM MYCOBACTERIUM TUBERCULOSIS COMPLEXED WITH NADPH AND PDC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 288 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-05-25 |
| Detector | XENTRONICS |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 118.831, 118.257, 79.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.400 - 2.390 |
| R-factor | 0.197 |
| Rwork | 0.193 |
| R-free | 0.24300 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.452 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.630 |
| High resolution limit [Å] | 2.400 | 2.540 |
| Rmerge | 0.105 | 0.395 |
| Number of reflections | 16553 | |
| <I/σ(I)> | 7.11 | |
| Completeness [%] | 87.6 | 88.68 |
| Redundancy | 3.29 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | ammonium sulfate, PEG400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |
