1C1Y

CRYSTAL STRUCTURE OF RAP.GMPPNP IN COMPLEX WITH THE RAS-BINDING-DOMAIN OF C-RAF1 KINASE (RAFRBD).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	1994-09-13
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	44.170, 71.880, 100.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.229
Rwork	0.229
R-free	0.25700
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.038	0.325
Number of reflections	24787
<I/σ(I)>	12
Completeness [%]	98.0	93.6
Redundancy	4.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	300	mmePeg5000, magnesium chloride, calcium chloride, ammonium sulphate., pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
10	1	reservoir		10 (mM)
11	1	reservoir		5 (mM)
12	1	reservoir	DTE	1 (mM)
13	1	reservoir	Tris-HCl	64.4 (mM)
2	1	drop	mPEG5000	10 (%)
3	1	drop	ammonium sulfate	50 (mM)
4	1	drop		5 (mM)
5	1	drop		2.5 (mM)
6	1	drop	DTE	0.5 (mM)
7	1	drop	Tris-HCl	32.2 (mM)
8	1	reservoir	mPEG5000	20 (%)
9	1	reservoir	ammonium sulfate	0.1 (M)