1C1B
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH GCA-186
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-15 |
Detector | FUJI |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 140.000, 111.500, 73.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.191 |
Rwork | 0.198 |
R-free | 0.25500 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.153 | 0.673 |
Total number of observations | 179492 * | |
Number of reflections | 37000 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 91.8 | 68.4 |
Redundancy | 4.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 4 * | Stammers, D.K., (1994) J.Mol.Biol., 242, 586. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 26 (mg/ml) | |
2 | 1 | drop | PEG3400 | 6 (%(w/v)) | |
3 | 1 | reservoir | PEG3400 | 6 (%(w/v)) | |
4 | 1 | drop | citrate/phosphate |