1C0K

CRYSTAL STRUCTURE ANALYSIS OF D-AMINO ACID OXIDASE IN COMPLEX WITH L-LACTATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-09-13
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	120.670, 120.670, 136.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 1.460
R-factor	0.112 *
R-free	0.16100 *
RMSD bond length	0.008
RMSD bond angle	1.730
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.460
Rmerge	0.053	0.288 *
Total number of observations	515122 *
Number of reflections	83893 *
<I/σ(I)>	20.1
Completeness [%]	99.1	91.2 *
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	18 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	PEG10000	15 (%)
4	1	reservoir	ammonium sulfate	200 (mM)
5	1	reservoir	L-lactate	1 (mM)