1BY3
FHUA FROM E. COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-29 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.900, 89.900, 90.000 |
Unit cell angles | 90.00, 95.20, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.740 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 27.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.740 |
Rmerge | 0.055 |
Number of reflections | 26534 |
Completeness [%] | 95.6 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.2 | protein solution is mixed in a 1:2 ratio with well solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12.3 (mg/ml) | |
2 | 1 | reservoir | PEG2000 | 33 (%) | |
3 | 1 | reservoir | 0.45 (M) | ||
4 | 1 | reservoir | 0.15 (M) | ||
5 | 1 | reservoir | n-octyl-2-hydroxyethyl-sulfoxide | 0.5 (%) |