1BWK
OLD YELLOW ENZYME (OYE1) MUTANT H191N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Detector | SDMS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 143.300, 143.300, 43.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.25400 |
Structure solution method | OTHER |
Starting model (for MR) | 1oya |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.084 * | 0.383 * |
Total number of observations | 76546 * | |
Number of reflections | 20012 | |
<I/σ(I)> | 12.3 | 2.4 |
Completeness [%] | 97.1 | 96.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 4 * | Fox, K.M., (1993) J. Mol. Biol., 234, 502. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | phrnylmethylsulfonyl fluoride | 0.01 (M) | |
5 | 1 | reservoir | HEPES | 0.1 (M) | |
6 | 1 | reservoir | PEG400 | 30 (%(v/v)) | |
7 | 1 | reservoir | 0.2 (M) |