1BWD

INOSAMINE-PHOSPHATE AMIDINOTRANSFERASE STRB1 FROM STREPTOMYCES GRISEUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	283
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43
Unit cell lengths	121.300, 121.300, 63.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.100
Rwork	0.197
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1JDW
RMSD bond length	0.009
RMSD bond angle	2.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.144 *	0.345 *
Total number of observations	37728 *
Number of reflections	14273
Completeness [%]	85.6	75.3
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5	20 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.3 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	glutathione	1 (mM)
4	1	drop	EDTA	0.2 (mM)
5	1	reservoir	PEG4000	20 (%(w/v))
6	1	reservoir	sodium citrate	0.1 (M)
7	1	reservoir	ammonium acetate	0.2 (M)