1BVO

DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-10-16
Detector	MAR scanner 345 mm plate
Spacegroup name	P 43 2 2
Unit cell lengths	87.610, 87.610, 96.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.219
Rwork	0.219
R-free	0.28800
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.041 *	0.286 *
Total number of observations	33776 *
Number of reflections	8927
<I/σ(I)>	18.9	2.7
Completeness [%]	82.9	88.5
Redundancy	3.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	21 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	2-5 (%)
2	1	reservoir	MES	50 (mM)
3	1	reservoir		5 (mM)
4	1	reservoir	dithiothreitol	3 (mM)