1BU2
X-RAY STRUCTURE OF A VIRAL CYCLIN FROM HERPESVIRUS SAIMIRI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Collection date | 1995-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.920, 61.440, 130.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.32400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 19.700 * |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.095 * | 0.350 * |
| Total number of observations | 23083 * | |
| Number of reflections | 6501 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 98.9 | 99.8 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 22 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.0-4.5 (mg/ml) | |
| 2 | 1 | drop | EDTA | 0.2 (mM) | |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | Na/K-phosphate | 0.8 (M) | |
| 5 | 1 | reservoir | PEG1500 | 4 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 20 (mM) |
