1BU1
SRC FAMILY KINASE HCK SH3 DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 280 |
| Detector technology | CCD |
| Collection date | 1996-11-15 |
| Detector | PRINCETON / THOMSON |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.500, 106.150, 78.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hck |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.810 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.053 | 0.189 |
| Number of reflections | 13043 | |
| <I/σ(I)> | 35 | |
| Completeness [%] | 93.0 | 62 * |
| Redundancy | 4.8 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 21 * | HANGING DROPS (2UL) OF 4.3MG/ML PROTEIN WERE MIXED WITH EQUAL VOLUMES OF RESERVOIR BUFFER CONTAINING 3.7 M SODIUM FORMATE, 2% PEG 3000, 100 MM BICINE (PH 9.3). THE MIXED DROPS WERE STORED AT 21 DEGREES, vapor diffusion - hanging drop, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.3 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | EGTA | 1 (mM) | |
| 5 | 1 | reservoir | Bicine | 100 (mM) | |
| 6 | 1 | reservoir | sodium formate | 3.7 (M) | |
| 7 | 1 | reservoir | PEG3000 | 2 (%) |
