1BTG

CRYSTAL STRUCTURE OF BETA NERVE GROWTH FACTOR AT 2.5 A RESOLUTION IN C2 SPACE GROUP WITH ZN IONS BOUND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	rotating-anode X-ray tube
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1993-01
Detector	AREA DETECTOR
Spacegroup name	C 1 2 1
Unit cell lengths	109.800, 93.900, 58.900
Unit cell angles	90.00, 123.10, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.179
RMSD bond length	0.014
RMSD bond angle	2.400
Data reduction software	XENGEN ((HOWARD)
Data scaling software	Xengen (HOWARD, NIELSEN, XUONG)
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.050
Total number of observations	44269 *
Number of reflections	15194 *
Completeness [%]	89.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.1 *		seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	PEG8000	18-20 (%)
3	1	reservoir	PIPES	100 (mM)
4	1	reservoir	zinc acetate	100 (mM)