1BSV

GDP-FUCOSE SYNTHETASE FROM ESCHERICHIA COLI COMPLEX WITH NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1997-07-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 32 2 1
Unit cell lengths	104.300, 104.300, 74.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.000 - 2.200
R-factor	0.17
R-free	0.20000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gfs
RMSD bond length	0.009
RMSD bond angle	1.380
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.056
Number of reflections	24049
<I/σ(I)>	26.4	4.4
Completeness [%]	99.6	96.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		4.0 M SODIUM FORMATE, pH 7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	sodium chloride	50 (mM)
4	1	reservoir	sodium formate	4.0 (M)