1BSP
THERMOSTABLE THYMIDYLATE SYNTHASE A FROM BACILLUS SUBTILIS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.223, 97.438, 118.019 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.26000 |
| Structure solution method | MIR/MR |
| Starting model (for MR) | HOMOLOGY MODEL BASED ON 1TJS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.095 | 0.312 |
| Total number of observations | 131066 * | |
| Number of reflections | 31172 | |
| <I/σ(I)> | 11.4 | 3.1 |
| Completeness [%] | 90.8 | 88.8 |
| Redundancy | 4.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 22 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | EDTA | 0.1 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) |
