1BQY

Plasminogen activator (TSV-PA) from snake venom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.290, 67.420, 163.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.178
Rwork	0.178
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ptb
RMSD bond length	0.009
RMSD bond angle	1.870
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.102	0.444
Total number of observations	91942 *
Number of reflections	17988
Completeness [%]	97.7	82.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	reservoir	Na acetate	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir	PEG4000	25 (%)