1BQN
TYR 188 LEU HIV-1 RT/HBY 097
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 1996-03 |
| Detector | PRINCETON 2K |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.800, 69.300, 105.300 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.300 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.36200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR (3.85) |
| Refinement software | X-PLOR (3.85) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.103 | 0.900 |
| Number of reflections | 29728 | |
| <I/σ(I)> | 13.14 | 2.7 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | inhibitor | 20 (mM) | |
| 10 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 2 | 1 | drop | DMSO | ||
| 3 | 1 | drop | n-octyl beta-D-glucopyranoside | 20 (%(w/v)) | |
| 4 | 1 | drop | enzyme | 40 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | drop | 75 (mM) | ||
| 7 | 1 | reservoir | bis-Tris propane | 50 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 9 | 1 | reservoir | glycerol | 10 (%(v/v)) |
