1BPZ

HUMAN DNA POLYMERASE BETA COMPLEXED WITH NICKED DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-11-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	53.535, 78.656, 54.618
Unit cell angles	90.00, 107.54, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.243
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9icj
RMSD bond length	0.020
RMSD bond angle	3.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MERLOT
Refinement software	TNT (5D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.073 *
Total number of observations	42489 *
Number of reflections	14702
Completeness [%]	94.0	95.6
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	DNA		0.005ml
2	1	2	PEG3350	17.5 (%)
3	1	2	sodium acetate	90 (mM)
4	1	2	imidazol	50 (mM)