1BP1
CRYSTAL STRUCTURE OF BPI, THE HUMAN BACTERICIDAL PERMEABILITY-INCREASING PROTEIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 1994-02 |
Detector | RIGAKU |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.600, 33.000, 85.200 |
Unit cell angles | 90.00, 101.60, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.29500 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 26.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.072 * | |
Number of reflections | 18908 | |
<I/σ(I)> | 2.6 | |
Completeness [%] | 92.7 | 94.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 | CRYSTALLIZED IN 12% PEG 8000, 0.2 M MG ACETATE, 0.1 NA CACODYLATE PH 6.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 12 (%(w/v)) | |
3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
4 | 1 | reservoir | sodium cacodylate | 100 (mM) |