1BOB

HISTONE ACETYLTRANSFERASE HAT1 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH ACETYL COENZYME A

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-02
Detector	BRANDEIS
Wavelength(s)	0.93, 0.9789
Spacegroup name	C 1 2 1
Unit cell lengths	111.300, 47.900, 75.700
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	28.900 - 2.300
R-factor	0.204
Rwork	0.204
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	23.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.034 *	0.139 *
Total number of observations	193352 *
Number of reflections	18259 *
<I/σ(I)>	20	5.29
Completeness [%]	97.5	88.3
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6	4 *	pH 7.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop			2-fold molar excess
3	1	drop	HEPES	2.5 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	drop		0.1 (M)
6	1	reservoir	PEG400	12 (%(w/v))
7	1	reservoir	Tris-Cl	0.1 (M)