1BOB
HISTONE ACETYLTRANSFERASE HAT1 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH ACETYL COENZYME A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.93, 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.300, 47.900, 75.700 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 28.900 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.034 * | 0.139 * |
| Total number of observations | 193352 * | |
| Number of reflections | 18259 * | |
| <I/σ(I)> | 20 | 5.29 |
| Completeness [%] | 97.5 | 88.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 | 4 * | pH 7.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 2-fold molar excess | ||
| 3 | 1 | drop | HEPES | 2.5 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | 0.1 (M) | ||
| 6 | 1 | reservoir | PEG400 | 12 (%(w/v)) | |
| 7 | 1 | reservoir | Tris-Cl | 0.1 (M) |
