1BM7
HUMAN TRANSTHYRETIN (PREALBUMIN) COMPLEX WITH FLUFENAMIC ACID (2-[[3-(TRIFLUOROMETHYL)PHENYL]AMINO] BENZOIC ACID)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-15 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.180, 85.320, 64.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.285 |
| Total number of observations | 86716 * | |
| Number of reflections | 16664 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | phosphate | 100 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 1 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) |
