1BL4
FKBP MUTANT F36V COMPLEXED WITH REMODELED SYNTHETIC LIGAND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.730, 41.850, 43.590 |
| Unit cell angles | 62.61, 82.75, 85.29 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fkf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.980 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | |
| Number of reflections | 16490 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 94.8 | 91 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | VAPOR DIFFUSION IN HANGING DROPS WITH 40 MG/ML COMPLEX AND 1.2M AMMONIUM SULFATE, 0.1M SODIUM PHOSPHATE, PH 6.0 OVER RESERVOIRS OF 2.4 M AMMONIUM SULFATE, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.2 (M) | |
| 3 | 1 | drop | sodium phosphate | 0.1 (M) | |
| 4 | 1 | drop | dithiothreitol | 20 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.4 (M) |
