1BJ4
RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 154.400, 154.400, 235.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.650 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.22600 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.680 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.102 | 0.659 * |
Total number of observations | 500988 * | |
Number of reflections | 49366 | |
<I/σ(I)> | 22.2 | 1.9 |
Completeness [%] | 97.8 | 25.9 * |
Redundancy | 10.1 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 286 * | Renwick, S.B., (1998) Acta Cryst., D54, 1030. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 22-27 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 1.0 (M) | |
3 | 1 | reservoir | imidazole | 0.1 (M) |