1BJ1
VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH A NEUTRALIZING ANTIBODY
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-03 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.860, 66.980, 140.510 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2VPF AND 1FVD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 27.970 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.128 |
| Number of reflections | 63147 | |
| <I/σ(I)> | 8.6 | 3.5 |
| Completeness [%] | 96.0 | 81.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.8 (A280) | |
| 2 | 1 | drop | Tris-HCl | 30 (mM) | |
| 3 | 1 | drop | sodium chloride | 0.4 (M) | |
| 4 | 1 | reservoir | PEG | 15 (%) | |
| 5 | 1 | reservoir | isopropanol | 10 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | reservoir | MES | 0.2 (M) |
