1BIY

STRUCTURE OF DIFERRIC BUFFALO LACTOFERRIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1996-05
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	56.797, 101.436, 76.275
Unit cell angles	90.00, 104.88, 90.00

Refinement procedure

Resolution	17.000 - 3.370
R-factor	0.218
Rwork	0.218
R-free	0.29600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HUMAN DIFERRIC LACTOFERRIN
RMSD bond length	0.010
RMSD bond angle	27.200 *
Data reduction software	MARSCALE
Data scaling software	MARSCALE
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	3.580
High resolution limit [Å]	3.300 *	3.300 *
Rmerge	0.051	0.093 *
Total number of observations	42104 *
Number of reflections	11711
<I/σ(I)>	34.47	13.35
Completeness [%]	98.5 *	60 *
Redundancy	3.68	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8	277 *	pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	50 (mg/ml)
2	1	1	Tris-HCl	0.025 (M)
3	1	2	ethanol	19 (%(v/v))
4	1	2	Tris-HCl	0.025 (M)