1BIY
STRUCTURE OF DIFERRIC BUFFALO LACTOFERRIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.797, 101.436, 76.275 |
| Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
| Resolution | 17.000 - 3.370 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.29600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN DIFERRIC LACTOFERRIN |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.200 * |
| Data reduction software | MARSCALE |
| Data scaling software | MARSCALE |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 3.580 |
| High resolution limit [Å] | 3.300 * | 3.300 * |
| Rmerge | 0.051 | 0.093 * |
| Total number of observations | 42104 * | |
| Number of reflections | 11711 | |
| <I/σ(I)> | 34.47 | 13.35 |
| Completeness [%] | 98.5 * | 60 * |
| Redundancy | 3.68 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8 | 277 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 50 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 0.025 (M) | |
| 3 | 1 | 2 | ethanol | 19 (%(v/v)) | |
| 4 | 1 | 2 | Tris-HCl | 0.025 (M) |
