1BIQ
RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 BETA CHAIN MUTANT E238A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.053, 83.780, 113.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.050 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.26600 * |
| Structure solution method | DIFFERENCE FOURIER WITH INITIAL REFINEMENT |
| Starting model (for MR) | DIFERROUS FORM OF R2 PROTEIN PDB ENTRY 1XIK |
| RMSD bond length | 0.014 |
| RMSD bond angle | 16.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.000 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.081 |
| Number of reflections | 161734 |
| <I/σ(I)> | 16.4 |
| Completeness [%] | 98.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | dioxane | 3 (%) | |
| 5 | 1 | reservoir | MES | 0.05 (M) |
