1BIF
6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE BIFUNCTIONAL ENZYME COMPLEXED WITH ATP-G-S AND PHOSPHATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-15 |
| Detector | RIGAKU |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.000, 83.000, 130.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.25000 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.032 * | |
| Number of reflections | 29479 | |
| <I/σ(I)> | 21.6 | 3.7 |
| Completeness [%] | 90.0 | 60.6 |
| Redundancy | 2 | 1.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | PROTEIN WAS CRYSTALLISED FROM 50MM SUCCINATE, PH 6.0, 10MM HEPES PH 7.0, 18% PEG 4000, 1% N-OCTYLGLUCOSIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | drop | reservoir solution | ||
| 11 | 1 | reservoir | PEG4000 | 15 (%) | |
| 12 | 1 | reservoir | succinate | 50 (mM) | |
| 13 | 1 | reservoir | HEPES | 10 (mM) | |
| 14 | 1 | reservoir | n-octylglucosude | 2 (%) | |
| 2 | 1 | drop | Tris-PO4 | 50 (mM) | |
| 3 | 1 | drop | glycerol | 5 (%) | |
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | PEG400 | 1 (%) | |
| 6 | 1 | drop | DTT | 10 (mM) | |
| 7 | 1 | drop | 3 (mM) | ||
| 8 | 1 | drop | ATP gamma S | 1 (mM) | |
| 9 | 1 | drop | F6P | 1 (mM) |
