1BHN
NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA
Experimental procedure
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1997-01-15 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.880, 92.110, 131.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.2 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PARTIALLY REFINED STRUCTURE OF THE B ISOFORM |
RMSD bond length | 0.021 |
RMSD bond angle | 20.460 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.510 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.122 * | 0.384 * |
Total number of observations | 165667 * | |
Number of reflections | 41872 | |
<I/σ(I)> | 6.8 | 1.4 |
Completeness [%] | 79.0 | 60 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | drop contains equal volume of the reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | (mg/ml) | |
2 | 1 | drop | GTP | (mM) | |
3 | 1 | drop | cGMP | (mM) | |
4 | 1 | reservoir | sodium acetate | (mM) | |
5 | 1 | reservoir | ammonium acetate | (mM) | |
6 | 1 | reservoir | PEG4000 | (%(w/v)) |