1BHN
NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-01-15 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.880, 92.110, 131.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.2 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIALLY REFINED STRUCTURE OF THE B ISOFORM |
| RMSD bond length | 0.021 |
| RMSD bond angle | 20.460 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.122 * | 0.384 * |
| Total number of observations | 165667 * | |
| Number of reflections | 41872 | |
| <I/σ(I)> | 6.8 | 1.4 |
| Completeness [%] | 79.0 | 60 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | drop contains equal volume of the reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | (mg/ml) | |
| 2 | 1 | drop | GTP | (mM) | |
| 3 | 1 | drop | cGMP | (mM) | |
| 4 | 1 | reservoir | sodium acetate | (mM) | |
| 5 | 1 | reservoir | ammonium acetate | (mM) | |
| 6 | 1 | reservoir | PEG4000 | (%(w/v)) |
