1BHF

P56LCK SH2 DOMAIN INHIBITOR COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-05-09
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	80.700, 45.200, 26.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.226
Rwork	0.226
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	RIGAKU
Data scaling software	RIGAKU
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.068
Total number of observations	19704 *
Number of reflections	8151

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		42 (mM)
2	1	drop	protein	18 (mg/ml)
3	1	reservoir	sodium cacodylate	100 (mM)	pH6.5
4	1	reservoir	PEG8000	30 (%)
5	1	reservoir	sodium acetate	200 (mM)