1BGQ
RADICICOL BOUND TO THE ATP BINDING SITE OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02 |
Detector | MARRESEARCH |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 73.940, 73.940, 110.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.193 * |
Rwork | 0.200 |
R-free | 0.26300 |
Structure solution method | ISOMORPHOUS REPLACEMENT |
Starting model (for MR) | 1ah6 |
RMSD bond length | 0.007 |
RMSD bond angle | 26.500 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.580 |
High resolution limit [Å] | 2.500 | 2.460 |
Rmerge | 0.073 * | 0.233 * |
Number of reflections | 11672 | |
<I/σ(I)> | 8.4 | 3.1 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 5 | 16 * | pH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 27 (mg/ml) | |
2 | 1 | 1 | mPEG550 | 9.75 (%(w/v)) | |
3 | 1 | 1 | ammonium sulfate | 65 (mM) | |
4 | 1 | 1 | sodium succinate | 32.5 (mM) | |
5 | 1 | 1 | glycerol | 25 (%(v/v)) |