1BG3
RAT BRAIN HEXOKINASE TYPE I COMPLEX WITH GLUCOSE AND INHIBITOR GLUCOSE-6-PHOSPHATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 153 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 132.100, 77.100, 137.100 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HEXOKINASE FROM SCHISTOSOMA MANSONI |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.600 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.076 | 0.170 |
| Number of reflections | 54454 | |
| <I/σ(I)> | 8 | 3 |
| Completeness [%] | 79.0 | 59 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 8 (%) | |
| 2 | 1 | reservoir | 0.13 (M) | ||
| 3 | 1 | reservoir | imidazole | 100 (mM) |
