1BE4

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1997-03
Detector	SIEMENS HI-STAR
Spacegroup name	P 43 21 2
Unit cell lengths	128.610, 128.610, 88.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nsk
RMSD bond length	0.024
RMSD bond angle	5.530
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.109 *
Number of reflections	30274
<I/σ(I)>	7.5	1.3
Completeness [%]	89.0	60
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		drops were formed by combining equal volumes of protein solution and reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cGMP	2.5 (mM)
2	1	reservoir	Tris-HCl	100 (mm)
3	1	reservoir	ammonium sulfate	2.3 (M)