1BDY

C2 DOMAIN FROM PROTEIN KINASE C DELTA

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1997-08
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	40.720, 60.690, 120.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.1976
Rwork	0.198
R-free	0.24730 *
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	1.317 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Rmerge	0.071 *
Total number of observations	36783 *
Number of reflections	13223
Completeness [%]	96.0
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6		Pappa, H.S., (1998) Acta Cryst., D54, 693. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000
2	1	drop	protein	5 (mg/ml)
3	1	reservoir	PEG4000