1BC8
STRUCTURES OF SAP-1 BOUND TO DNA SEQUENCES FROM THE E74 AND C-FOS PROMOTERS PROVIDE INSIGHTS INTO HOW ETS PROTEINS DISCRIMINATE BETWEEN RELATED DNA TARGETS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.590, 55.670, 44.230 |
Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
Resolution | 28.900 - 1.930 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.27700 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 23.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.900 | 2.020 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.071 | 0.141 |
Number of reflections | 10728 | |
<I/σ(I)> | 15 | |
Completeness [%] | 96.7 | 91.7 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 20 * | 50 MM NA CACODYLATE (PH 6.0), 5.0% PEG8000, 20 MM ZINC ACETATE |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CACODYLATE | ||
2 | 1 | 1 | PEG 8000 | ||
3 | 1 | 1 | ZINC ACETATE | ||
4 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium cacodylate | 25 (mM) | |
2 | 1 | drop | PEG8000 | 2.5 (%) | |
3 | 1 | drop | zinc acetate | 10 (mM) |