1B92
MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05 |
| Detector | MACSCIENCE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.260, 72.260, 65.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.020 |
| R-factor | 0.219 * |
| Rwork | 0.225 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b9d |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 0.037 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.039 * |
| Number of reflections | 13085 |
| <I/σ(I)> | 11.9 |
| Completeness [%] | 98.5 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 15 (%) | |
| 2 | 1 | reservoir | cacodylic acid | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | 5 (mM) | ||
| 6 | 1 | reservoir | 5 (mM) |
