1B8U
MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 83 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.300, 101.700, 52.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
Rwork | 0.198 |
R-free | 0.23400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | APO MDH FROM A. ARCTICUM |
RMSD bond length | 0.010 |
RMSD bond angle | 1.650 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.090 |
Number of reflections | 9952 |
<I/σ(I)> | 23.7 |
Completeness [%] | 87.4 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 18 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
3 | 1 | reservoir | sodium acetate | 400 (mM) | |
4 | 1 | reservoir | PEG4000 | 35 (%sat) |