1B7U
Structure of Mare Apolactoferrin: the N and C Lobes are in the Closed Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.400, 77.400, 102.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.000 - 3.800 |
Rwork | 0.200 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DIFERRIC MARE LACTOFERRIN |
RMSD bond length | 0.016 |
RMSD bond angle | 24.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | |
High resolution limit [Å] | 3.800 | 3.800 * |
Rmerge | 0.150 | |
Total number of observations | 101096 * | |
Number of reflections | 7887 | |
<I/σ(I)> | 14 | |
Completeness [%] | 99.2 | 98.6 * |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 8.5 | 277 * | pH 8.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | TRIS-HCL | ||
2 | 1 | 2 | METHANOL | ||
3 | 1 | 2 | TRIS-HCL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | protein | 40-50 (mg/ml) | ||
2 | 1 | 1 | Tris-HCl | 0.01 (M) | |
3 | 1 | Tris-HCl | 0.01 (M) | ||
4 | 1 | 2 | ethanol | 10 (%(v/v)) |