1B74

GLUTAMATE RACEMASE FROM AQUIFEX PYROPHILUS

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Wavelength(s)	1.000, 1.015, 1.1
Spacegroup name	P 65 2 2
Unit cell lengths	72.170, 72.170, 184.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.221
R-free	0.28600
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	24.800 *
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.300
Rmerge	0.056
Number of reflections	11546
Completeness [%]	88.4
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	18 *	pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	PEG6000	6 (%(w/v))