1B72
PBX1, HOMEOBOX PROTEIN HOX-B1/DNA TERNARY COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.670, 64.580, 81.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.350 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.27700 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 16.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.033 * | 0.248 * |
Number of reflections | 13735 | |
<I/σ(I)> | 27.2 | 7.7 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | equal volume of protein and reservoir solution were used for the drop * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | TRIS | ||
2 | 1 | 1 | PEG 1000 | ||
3 | 1 | 1 | CO(NH3)6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | |
2 | 1 | drop | EDTA | 1 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | 0.001 (%) | ||
5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
6 | 1 | reservoir | cobaltic hexamine chloride | 5 (mM) | |
7 | 1 | reservoir | PEG1000 | 12 (%) | |
8 | 1 | drop | protein-DNA comples | protein was mixed with lyophilized DNA in a molar ration of 1:1:1.5 HoxB1:Pbx1:DNA |