1B6Z

6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Spacegroup name	P 3 2 1
Unit cell lengths	121.500, 121.500, 61.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.216
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gtq
RMSD bond length	0.011
RMSD bond angle	24.700 *
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000	2.000 *
Rmerge	0.066	0.320 *
Total number of observations	111420 *
Number of reflections	32839
Completeness [%]	92.6	94.6 *
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	4 *	1.6 M AMMONIUM SULFATE 0.1 M TRIS/HCL, PH 9.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	Mes/NaOH	1 (mM)
3	1	reservoir	ammonium sulfate	1.3 (M)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir		2 (mM)