1B6A

HUMAN METHIONINE AMINOPEPTIDASE 2 COMPLEXED WITH TNP-470

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-08
Detector	ADSC
Spacegroup name	C 2 2 21
Unit cell lengths	89.558, 99.060, 101.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.187
Rwork	0.187
R-free	0.21600
Starting model (for MR)	1bn5
RMSD bond length	0.009
RMSD bond angle	24.900 *
Data reduction software	SOFTWARE (AT SYNCHROTRON)
Data scaling software	SCALA
Phasing software	CNS (0.4)
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.670
High resolution limit [Å]	1.600	1.580
Rmerge	0.080	0.231
Number of reflections	59207
<I/σ(I)>	11.7	4.3
Completeness [%]	96.0	85.8
Redundancy	4.0 *	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4		pH 5.40