1B56

HUMAN RECOMBINANT EPIDERMAL FATTY ACID BINDING PROTEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	63.450, 63.450, 76.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.050
R-factor	0.207
Rwork	0.207
R-free	0.26500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hmb
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.070	0.275
Total number of observations	72226 *
Number of reflections	10321
<I/σ(I)>	20	12
Completeness [%]	99.7	100
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2 (M)
2	1	reservoir	sodium potassium tartrate	0.2 (M)
3	1	reservoir	citrate	0.1 (M)