1B4P

CRYSTAL STRUCTURES OF CLASS MU CHIMERIC GST ISOENZYMES M1-2 AND M2-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1996-04-15
Detector	BRUKER
Spacegroup name	C 2 2 21
Unit cell lengths	73.590, 82.550, 79.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.179
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1GST
RMSD bond length	0.022
RMSD bond angle	0.042
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	GPRLSA

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.810
High resolution limit [Å]	1.700	1.700
Number of reflections	27013
<I/σ(I)>	8.6	1.9
Completeness [%]	98.3	89.7
Redundancy	8.6	36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		PROTEIN CONC. 10 MG/ML, 25 MM TRIS BUFFER (PH 8.0), 1 MM EDTA, 0.3% OCTYL BETA- D-GLUCOPYRANOSIDE, 2 MM PRODUCT INHIBITOR (GPS)