1AY9
WILD-TYPE UMUD' FROM E. COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1990-12 |
| Detector | FUJI |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.100, 53.100, 164.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 4912 |
| Completeness [%] | 90.0 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 * | 20 * | 100MM CACODYLATE BUFFER PH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cacodylate | 100 (mM) | |
| 2 | 1 | reservoir | 600 (mM) | ||
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | free DL-methionine | 2 (mg/ml) | |
| 6 | 1 | drop | protein | 12-15 (mg/ml) |
