1AVS
X-RAY CRYSTALLOGRAPHIC STUDY OF CALCIUM-SATURATED N-TERMINAL DOMAIN OF TROPONIN C
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.120, 81.580, 39.860 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.25100 * |
| Structure solution method | MOLECULAR REPLACEMENT AND MIR |
| Starting model (for MR) | 4tnc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 18.460 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.052 | 0.050 |
| Total number of observations | 85689 * | |
| Number of reflections | 18010 | |
| <I/σ(I)> | 15 | 3 |
| Completeness [%] | 82.0 | 82 |
| Redundancy | 4.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 72 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | MPD | 2 (%(v/v)) |
