1AVC
BOVINE ANNEXIN VI (CALCIUM-BOUND)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-14 |
| Detector | FUJI |
| Spacegroup name | P 43 |
| Unit cell lengths | 67.404, 67.404, 200.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.204 * |
| Rwork | 0.205 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BOVINE ANNEXIN VI: PDB ENTRY 1ANN |
| RMSD bond length | 0.003 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 2.600 |
| High resolution limit [Å] | 2.900 * | 2.900 * |
| Rmerge | 0.050 * | 0.137 * |
| Number of reflections | 19075 * | 1912 * |
| <I/σ(I)> | 20.7 | 4.6 |
| Completeness [%] | 98.7 * | 99.6 * |
| Redundancy | 3.8 | 3.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 * | VAPOUR DIFFUSION (HANGING DROP), SODIUM ACETATE 1.2 M AS PRECIPITANT, POTASSIUM CACODYLATE 0.1 M, PH 6.5, CALCIUM CHLORIDE 6.2 MM ROOM TEMPERATURE, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | Na acetate | 0.2 (M) | |
| 3 | 1 | drop | Na-cacodylate | 0.02 (M) | |
| 4 | 1 | drop | 1.04 (mM) | ||
| 5 | 1 | drop | 0.11 (mM) | ||
| 6 | 1 | reservoir | Na-acetate | 1.2 (M) | |
| 7 | 1 | reservoir | Na-cacodylate | 0.1 (M) | |
| 8 | 1 | reservoir | 6.25 (mM) | ||
| 9 | 1 | reservoir | 0.660 (mM) |
