1AVC

BOVINE ANNEXIN VI (CALCIUM-BOUND)

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-04-14
Detector	FUJI
Spacegroup name	P 43
Unit cell lengths	67.404, 67.404, 200.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.204 *
Rwork	0.205
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BOVINE ANNEXIN VI: PDB ENTRY 1ANN
RMSD bond length	0.003
RMSD bond angle	22.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.600
High resolution limit [Å]	2.900 *	2.900 *
Rmerge	0.050 *	0.137 *
Number of reflections	19075 *	1912 *
<I/σ(I)>	20.7	4.6
Completeness [%]	98.7 *	99.6 *
Redundancy	3.8	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7 *		VAPOUR DIFFUSION (HANGING DROP), SODIUM ACETATE 1.2 M AS PRECIPITANT, POTASSIUM CACODYLATE 0.1 M, PH 6.5, CALCIUM CHLORIDE 6.2 MM ROOM TEMPERATURE, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	Na acetate	0.2 (M)
3	1	drop	Na-cacodylate	0.02 (M)
4	1	drop		1.04 (mM)
5	1	drop		0.11 (mM)
6	1	reservoir	Na-acetate	1.2 (M)
7	1	reservoir	Na-cacodylate	0.1 (M)
8	1	reservoir		6.25 (mM)
9	1	reservoir		0.660 (mM)